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The fungal metabolite epichlorohydrin (EpiCHO) is required for the induction of microsomal drug-metabolizing enzymes (xenobiotic epoxide-forming enzymes). The nature of the xenobiotic-metabolizing activities induced by a variety of dietary and environmental chemicals has been studied in hepatic and intestinal microsomes from various strains of rodents and other mammals. Microsomes of EpiCHO-induced animals exhibit a characteristic induction of microsomal glutathione-S-transferase activity and higher levels of total cytochrome P450. Hepatic microsomes isolated from 6-week-old EpiCHO-induced rats are approximately fivefold more active than microsomes from untreated rats in the dealkylation of 1-chloro-2,4-dinitrobenzene to form an epoxide product (oxiran). 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